ssr_primer
Primer Details for NODE_381100_length_353_cov_2.157143 |
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| (G)13tgggggga(G)66agcggggacagggggagggggaaagagcaaaagacaagaaagaaacag(A)27: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCCACACCAACCCTCTTT | TGATGTGGGATGATCTGGCG | Start: 7 |
| Tm | 60.106 | 59.893 | End: 116 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
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| Sequence | CTCTTCCGATCTGTGGGACG | TTTTGCTCTTTCCCCCTCCC | Start: 39 |
| Tm | 59.899 | 59.886 | End: 308 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
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| Sequence | CGCCAGATCATCCCACATCA | TTTTGCTCTTTCCCCCTCCC | Start: 97 |
| Tm | 59.893 | 59.886 | End: 308 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
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| Sequence | CTCTTCCGATCTGTGGGACG | CCCTCTTTCCCTACACGACG | Start: 39 |
| Tm | 59.899 | 59.827 | End: 181 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
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| Sequence | CACCCACACCAACCCTCTTT | CCCTCTTTCCCTACACGACG | Start: 7 |
| Tm | 60.106 | 59.827 | End: 181 |
| Size | 20 | 20 | 175 |
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