Primer Details for NODE_380279_length_227_cov_1.285714 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACGCCATTTTCCTACGGA | GTCCATCTTATGCACATTAACGTGT | Start: 53 |
| Tm | 59.678 | 59.935 | End: 226 |
| Size | 20 | 25 | 174 |
| Primer 2 details |
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| Sequence | ACCACAACACGCCATTTTCC | GTCCATCTTATGCACATTAACGTGT | Start: 47 |
| Tm | 59.612 | 59.935 | End: 226 |
| Size | 20 | 25 | 180 |
| Primer 3 details |
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| Sequence | ACACGCCATTTTCCTACGGA | TCCATCTTATGCACATTAACGTGT | Start: 53 |
| Tm | 59.678 | 58.818 | End: 225 |
| Size | 20 | 24 | 173 |
| Primer 4 details |
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| Sequence | ACCACAACACGCCATTTTCC | TCCATCTTATGCACATTAACGTGT | Start: 47 |
| Tm | 59.612 | 58.818 | End: 225 |
| Size | 20 | 24 | 179 |
| Primer 5 details |
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| Sequence | ACGCCATTTTCCTACGGACT | GTCCATCTTATGCACATTAACGTGT | Start: 55 |
| Tm | 59.388 | 59.935 | End: 226 |
| Size | 20 | 25 | 172 |
