Primer Details for NODE_376554_length_208_cov_0.911111 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGAGAAACTTGTTTTGTTTTGTTT | AAACAAAACAAAAACAGCAAATTGTT | Start: 63 |
| Tm | 57.568 | 57.36 | End: 203 |
| Size | 26 | 26 | 141 |
| Primer 2 details |
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| Sequence | AGTGAGAAACTTGTTTTGTTTTGTTT | AAAACAAAACAAAAACAGCAAATTGT | Start: 63 |
| Tm | 57.568 | 57.36 | End: 204 |
| Size | 26 | 26 | 142 |
| Primer 3 details |
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| Sequence | TGAGAAACTTGTTTTGTTTTGTTTTGT | AAACAAAACAAAAACAGCAAATTGTT | Start: 65 |
| Tm | 58.331 | 57.36 | End: 203 |
| Size | 27 | 26 | 139 |
| Primer 4 details |
|---|
| Sequence | TGAGAAACTTGTTTTGTTTTGTTTTGT | AAAACAAAACAAAAACAGCAAATTGT | Start: 65 |
| Tm | 58.331 | 57.36 | End: 204 |
| Size | 27 | 26 | 140 |
| Primer 5 details |
|---|
| Sequence | AGTGAGAAACTTGTTTTGTTTTGTTT | AAAACAAAACAAAAACAGCAAATTGTT | Start: 63 |
| Tm | 57.568 | 57.908 | End: 204 |
| Size | 26 | 27 | 142 |
