Primer Details for NODE_375529_length_264_cov_2.963351 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTTTACCTTTCATTCTAGCCT | ACTAGGCAAAAAGATTTTCGAATGT | Start: 81 |
| Tm | 58.199 | 58.018 | End: 231 |
| Size | 24 | 25 | 151 |
| Primer 2 details |
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| Sequence | CGTTTTACCTTTCATTCTAGCCTTCA | ACTAGGCAAAAAGATTTTCGAATGT | Start: 82 |
| Tm | 59.851 | 58.018 | End: 231 |
| Size | 26 | 25 | 150 |
| Primer 3 details |
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| Sequence | TCGTTTTACCTTTCATTCTAGCCTT | ACTAGGCAAAAAGATTTTCGAATGT | Start: 81 |
| Tm | 58.764 | 58.018 | End: 231 |
| Size | 25 | 25 | 151 |
| Primer 4 details |
|---|
| Sequence | TTCGTTTTACCTTTCATTCTAGCCT | ACTAGGCAAAAAGATTTTCGAATGT | Start: 80 |
| Tm | 58.764 | 58.018 | End: 231 |
| Size | 25 | 25 | 152 |
| Primer 5 details |
|---|
| Sequence | TCGTTTTACCTTTCATTCTAGCCT | ACTAGGCAAAAAGATTTTCGAATGTT | Start: 81 |
| Tm | 58.199 | 58.566 | End: 231 |
| Size | 24 | 26 | 151 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTTTACCTTTCATTCTAGCCT | ACTAGGCAAAAAGATTTTCGAATGT | Start: 81 |
| Tm | 58.199 | 58.018 | End: 231 |
| Size | 24 | 25 | 151 |
| Primer 2 details |
|---|
| Sequence | CGTTTTACCTTTCATTCTAGCCTTCA | ACTAGGCAAAAAGATTTTCGAATGT | Start: 82 |
| Tm | 59.851 | 58.018 | End: 231 |
| Size | 26 | 25 | 150 |
| Primer 3 details |
|---|
| Sequence | TCGTTTTACCTTTCATTCTAGCCTT | ACTAGGCAAAAAGATTTTCGAATGT | Start: 81 |
| Tm | 58.764 | 58.018 | End: 231 |
| Size | 25 | 25 | 151 |
| Primer 4 details |
|---|
| Sequence | TTCGTTTTACCTTTCATTCTAGCCT | ACTAGGCAAAAAGATTTTCGAATGT | Start: 80 |
| Tm | 58.764 | 58.018 | End: 231 |
| Size | 25 | 25 | 152 |
| Primer 5 details |
|---|
| Sequence | TCGTTTTACCTTTCATTCTAGCCT | ACTAGGCAAAAAGATTTTCGAATGTT | Start: 81 |
| Tm | 58.199 | 58.566 | End: 231 |
| Size | 24 | 26 | 151 |
