Primer Details for NODE_368056_length_307_cov_1.303419 |
|---|
| (AAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTAGGATCCCACACCCA | TGCAAAGATTTCATTCAGGTATGT | Start: 187 |
| Tm | 59.58 | 57.161 | End: 290 |
| Size | 20 | 24 | 104 |
| Primer 2 details |
|---|
| Sequence | CCTTAGGATCCCACACCCAC | TGCAAAGATTTCATTCAGGTATGT | Start: 188 |
| Tm | 59.453 | 57.161 | End: 290 |
| Size | 20 | 24 | 103 |
| Primer 3 details |
|---|
| Sequence | ACGGGCACAAATTATAGAATCATACA | TGGGTGTGGGATCCTAAGGA | Start: 0 |
| Tm | 59.004 | 59.58 | End: 206 |
| Size | 26 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | ACGGGCACAAATTATAGAATCATACA | GTGGGTGTGGGATCCTAAGG | Start: 0 |
| Tm | 59.004 | 59.453 | End: 207 |
| Size | 26 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | TCCTTAGGATCCCACACCCA | TTGCAAAGATTTCATTCAGGTATGT | Start: 187 |
| Tm | 59.58 | 57.769 | End: 291 |
| Size | 20 | 25 | 105 |
