Primer Details for NODE_357904_length_240_cov_2.245509 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCGACGACTCCAACGTTT | AGATTCAAGTCTGAGATTCAGTCGA | Start: 3 |
| Tm | 59.899 | 59.583 | End: 113 |
| Size | 20 | 25 | 111 |
| Primer 2 details | |||
| Sequence | TGTCGACGACTCCAACGTTT | TCAAGTCTGAGATTCAGTCGAAA | Start: 3 |
| Tm | 59.899 | 57.546 | End: 109 |
| Size | 20 | 23 | 107 |
| Primer 3 details | |||
| Sequence | TGTCGACGACTCCAACGTTT | TTCAAGTCTGAGATTCAGTCGAA | Start: 3 |
| Tm | 59.899 | 57.546 | End: 110 |
| Size | 20 | 23 | 108 |
| Primer 4 details | |||
| Sequence | ATGTCGACGACTCCAACGTT | AGATTCAAGTCTGAGATTCAGTCGA | Start: 2 |
| Tm | 59.687 | 59.583 | End: 113 |
| Size | 20 | 25 | 112 |
| Primer 5 details | |||
| Sequence | TGTCGACGACTCCAACGTTT | GATTCAAGTCTGAGATTCAGTCGA | Start: 3 |
| Tm | 59.899 | 58.354 | End: 112 |
| Size | 20 | 24 | 110 |
