Primer Details for NODE_355758_length_224_cov_2.298013 | |||
|---|---|---|---|
| (A)15: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCCTGGAAGTATACACACA | TGCTCTTCTAGGGAACTATGTACC | Start: 51 |
| Tm | 58.807 | 59.106 | End: 200 |
| Size | 21 | 24 | 150 |
| Primer 2 details | |||
| Sequence | CCCCCTGGAAGTATACACACA | AGTTGCTCTTCTAGGGAACTATGT | Start: 51 |
| Tm | 58.807 | 58.973 | End: 203 |
| Size | 21 | 24 | 153 |
| Primer 3 details | |||
| Sequence | CCCCCTGGAAGTATACACACA | GCTCTTCTAGGGAACTATGTACCT | Start: 51 |
| Tm | 58.807 | 58.858 | End: 199 |
| Size | 21 | 24 | 149 |
| Primer 4 details | |||
| Sequence | CCCCCTGGAAGTATACACACAA | TGCTCTTCTAGGGAACTATGTACC | Start: 51 |
| Tm | 59.426 | 59.106 | End: 200 |
| Size | 22 | 24 | 150 |
| Primer 5 details | |||
| Sequence | CCCCCTGGAAGTATACACACA | TGCTCTTCTAGGGAACTATGTACCT | Start: 51 |
| Tm | 58.807 | 60.341 | End: 200 |
| Size | 21 | 25 | 150 |
