Primer Details for NODE_352561_length_261_cov_1.744681 | |||
|---|---|---|---|
| (A)10(T)(AT)5ccaacaagacaaagtaccaagtaacttggtattcgacacctgtggaagc(T)15aattatttattatatttaatataattcagtctgggttactagacgaac(T)17cttctttttatttttttttcgttgagttaaattaaacatat(A)18: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAACAAGACAAAGTACCAAGT | TCGTCTAGTAACCCAGACTGA | Start: 41 |
| Tm | 58.149 | 57.292 | End: 151 |
| Size | 23 | 21 | 111 |
| Primer 2 details | |||
| Sequence | TCCAACAAGACAAAGTACCAAGT | GTTCGTCTAGTAACCCAGACTGA | Start: 41 |
| Tm | 58.149 | 59.245 | End: 153 |
| Size | 23 | 23 | 113 |
| Primer 3 details | |||
| Sequence | TCCAACAAGACAAAGTACCAAGT | AGTTCGTCTAGTAACCCAGACTG | Start: 41 |
| Tm | 58.149 | 59.243 | End: 154 |
| Size | 23 | 23 | 114 |
| Primer 4 details | |||
| Sequence | TCCAACAAGACAAAGTACCAAGT | TCGTCTAGTAACCCAGACTGAA | Start: 41 |
| Tm | 58.149 | 57.975 | End: 151 |
| Size | 23 | 22 | 111 |
| Primer 5 details | |||
| Sequence | TCCAACAAGACAAAGTACCAAGT | TTCGTCTAGTAACCCAGACTGA | Start: 41 |
| Tm | 58.149 | 57.975 | End: 152 |
| Size | 23 | 22 | 112 |
