ssr_primer
Primer Details for NODE_349521_length_228_cov_3.974194 |
|---|
| (A)10tcattttgaattcttaaaaattagagatgaacaccaagaacccttttagcttctagaccttcaaaatgtgttccgattttatatttgatattcca(T)10gttttttgggcct(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGATGAACACCAAGAACCCT | GCGGTCGGAAAGTTTGTACTC | Start: 83 |
| Tm | 59.018 | 59.542 | End: 227 |
| Size | 22 | 21 | 145 |
| Primer 2 details |
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| Sequence | AGAGATGAACACCAAGAACCCT | GCGGTCGGAAAGTTTGTACT | Start: 83 |
| Tm | 59.018 | 58.495 | End: 227 |
| Size | 22 | 20 | 145 |
| Primer 3 details |
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| Sequence | AGAGATGAACACCAAGAACCCTT | GCGGTCGGAAAGTTTGTACTC | Start: 83 |
| Tm | 59.604 | 59.542 | End: 227 |
| Size | 23 | 21 | 145 |
| Primer 4 details |
|---|
| Sequence | GAGATGAACACCAAGAACCCT | GCGGTCGGAAAGTTTGTACTC | Start: 84 |
| Tm | 57.575 | 59.542 | End: 227 |
| Size | 21 | 21 | 144 |
| Primer 5 details |
|---|
| Sequence | AGAGATGAACACCAAGAACCC | GCGGTCGGAAAGTTTGTACTC | Start: 83 |
| Tm | 57.575 | 59.542 | End: 227 |
| Size | 21 | 21 | 145 |
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