Primer Details for NODE_345196_length_254_cov_0.895028 |
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| (T)24atgaaagaacaaaccccctcaacatataaactaccccccttgctccgccaccgggggggaaattatttatttgttg(T)34atgtttttttatg(T)27atgttttatttctccgactctttc(T)28: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAACAAACCCCCTCAACA | AAAGAAAGAGTCGGAGAAATAAAACA | Start: 31 |
| Tm | 57.161 | 57.553 | End: 203 |
| Size | 20 | 26 | 173 |
| Primer 2 details |
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| Sequence | AAGAACAAACCCCCTCAACA | AAGAAAGAGTCGGAGAAATAAAACAT | Start: 31 |
| Tm | 57.161 | 57.377 | End: 202 |
| Size | 20 | 26 | 172 |
| Primer 3 details |
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| Sequence | GAAAGAACAAACCCCCTCAACA | AAAGAAAGAGTCGGAGAAATAAAACA | Start: 29 |
| Tm | 58.972 | 57.553 | End: 203 |
| Size | 22 | 26 | 175 |
| Primer 4 details |
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| Sequence | TGAAAGAACAAACCCCCTCAAC | AAAGAAAGAGTCGGAGAAATAAAACA | Start: 28 |
| Tm | 58.972 | 57.553 | End: 203 |
| Size | 22 | 26 | 176 |
| Primer 5 details |
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| Sequence | AAAGAACAAACCCCCTCAACA | AAAGAAAGAGTCGGAGAAATAAAACA | Start: 30 |
| Tm | 57.891 | 57.553 | End: 203 |
| Size | 21 | 26 | 174 |
