Primer Details for NODE_344345_length_232_cov_1.213836 |
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| (T)11actgttatacaaagtgtgattaaagacttacacaaaagagctatgatctacagacgaaaattttcaatt(A)11gacaaggaaatgc(A)35: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAAAGAGCTATGATCTACAGACGA | CCTCCCCACCAGCAACAAAA | Start: 76 |
| Tm | 59.904 | 60.759 | End: 223 |
| Size | 26 | 20 | 148 |
| Primer 2 details |
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| Sequence | CACAAAAGAGCTATGATCTACAGACG | CCTCCCCACCAGCAACAAAA | Start: 75 |
| Tm | 59.802 | 60.759 | End: 223 |
| Size | 26 | 20 | 149 |
| Primer 3 details |
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| Sequence | ACAAAAGAGCTATGATCTACAGACG | CCTCCCCACCAGCAACAAAA | Start: 76 |
| Tm | 58.731 | 60.759 | End: 223 |
| Size | 25 | 20 | 148 |
| Primer 4 details |
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| Sequence | ACAAAAGAGCTATGATCTACAGACGA | CCTCCCCACCAGCAACAAA | Start: 76 |
| Tm | 59.904 | 60.153 | End: 223 |
| Size | 26 | 19 | 148 |
| Primer 5 details |
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| Sequence | CAAAAGAGCTATGATCTACAGACGA | CCTCCCCACCAGCAACAAAA | Start: 77 |
| Tm | 58.501 | 60.759 | End: 223 |
| Size | 25 | 20 | 147 |
