Primer Details for NODE_340412_length_290_cov_1.894009 |
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| (T)15catttcaattattattaactttttagctacatagctagatttgagttgtgcataacggcgattaaattaagcaaaatcttagttggtaaatttatacatc(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAATTATCACTAAAAATTAGCCCGT | TCGCCGTTATGCACAACTCA | Start: 10 |
| Tm | 57.814 | 60.32 | End: 143 |
| Size | 27 | 20 | 134 |
| Primer 2 details |
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| Sequence | AGAAATTATCACTAAAAATTAGCCCGT | CGCCGTTATGCACAACTCAA | Start: 10 |
| Tm | 57.814 | 59.485 | End: 142 |
| Size | 27 | 20 | 133 |
| Primer 3 details |
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| Sequence | AGAAATTATCACTAAAAATTAGCCCGT | ATCGCCGTTATGCACAACTC | Start: 10 |
| Tm | 57.814 | 58.993 | End: 144 |
| Size | 27 | 20 | 135 |
| Primer 4 details |
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| Sequence | AGAAATTATCACTAAAAATTAGCCCGT | CGCCGTTATGCACAACTCAAA | Start: 10 |
| Tm | 57.814 | 60.07 | End: 142 |
| Size | 27 | 21 | 133 |
| Primer 5 details |
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| Sequence | AGAAATTATCACTAAAAATTAGCCCGT | AATCGCCGTTATGCACAACTC | Start: 10 |
| Tm | 57.814 | 59.603 | End: 145 |
| Size | 27 | 21 | 136 |
