Primer Details for NODE_335515_length_355_cov_1.893617 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCGCGAGTCTTGTATGT | TGGATACATTTATAGCGCTGATTTT | Start: 5 |
| Tm | 59.756 | 57.256 | End: 165 |
| Size | 20 | 25 | 161 |
| Primer 2 details |
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| Sequence | AGCGCGAGTCTTGTATGTCT | TGGATACATTTATAGCGCTGATTTT | Start: 7 |
| Tm | 59.188 | 57.256 | End: 165 |
| Size | 20 | 25 | 159 |
| Primer 3 details |
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| Sequence | CAGCGCGAGTCTTGTATGTC | TGGATACATTTATAGCGCTGATTTT | Start: 6 |
| Tm | 59.083 | 57.256 | End: 165 |
| Size | 20 | 25 | 160 |
| Primer 4 details |
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| Sequence | AGCGCGAGTCTTGTATGTCTT | TGGATACATTTATAGCGCTGATTTT | Start: 7 |
| Tm | 59.797 | 57.256 | End: 165 |
| Size | 21 | 25 | 159 |
| Primer 5 details |
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| Sequence | ACTTTACAGCGCGAGTCTTGT | TGGATACATTTATAGCGCTGATTTT | Start: 0 |
| Tm | 60.27 | 57.256 | End: 165 |
| Size | 21 | 25 | 166 |
