Primer Details for NODE_333698_length_248_cov_2.714286 |
|---|
| (A)10gaaaacatttctctttatcaatggttgtagagataaatttaataattgtatg(A)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATTTCTCTTTATCAATGGTTGTAGA | AATTGATACAAGTGCATGGCGT | Start: 103 |
| Tm | 57.261 | 59.245 | End: 202 |
| Size | 27 | 22 | 100 |
| Primer 2 details |
|---|
| Sequence | ACATTTCTCTTTATCAATGGTTGTAGA | AATTGATACAAGTGCATGGCGTT | Start: 103 |
| Tm | 57.261 | 59.807 | End: 202 |
| Size | 27 | 23 | 100 |
| Primer 3 details |
|---|
| Sequence | ACATTTCTCTTTATCAATGGTTGTAGA | AAATTGATACAAGTGCATGGCGT | Start: 103 |
| Tm | 57.261 | 59.807 | End: 203 |
| Size | 27 | 23 | 101 |
| Primer 4 details |
|---|
| Sequence | ACATTTCTCTTTATCAATGGTTGTAGA | AATTGATACAAGTGCATGGCG | Start: 103 |
| Tm | 57.261 | 57.574 | End: 202 |
| Size | 27 | 21 | 100 |
| Primer 5 details |
|---|
| Sequence | ACATTTCTCTTTATCAATGGTTGTAGA | AAATTGATACAAGTGCATGGCG | Start: 103 |
| Tm | 57.261 | 58.227 | End: 203 |
| Size | 27 | 22 | 101 |
