Primer Details for NODE_333493_length_221_cov_2.128378 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGAGTGTCTCTTGACTTGACG | GCCTACTATAGCTCAATAGTGCTCA | Start: 17 |
| Tm | 59.996 | 59.816 | End: 220 |
| Size | 23 | 25 | 204 |
| Primer 2 details |
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| Sequence | AGAGTGTCTCTTGACTTGACGA | GCCTACTATAGCTCAATAGTGCTCA | Start: 19 |
| Tm | 58.785 | 59.816 | End: 220 |
| Size | 22 | 25 | 202 |
| Primer 3 details |
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| Sequence | GAGAGTGTCTCTTGACTTGACGA | GCCTACTATAGCTCAATAGTGCTCA | Start: 18 |
| Tm | 59.749 | 59.816 | End: 220 |
| Size | 23 | 25 | 203 |
| Primer 4 details |
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| Sequence | TGTCATAAATGCAAGGGCTTCA | GCCTACTATAGCTCAATAGTGCTCA | Start: 53 |
| Tm | 58.571 | 59.816 | End: 220 |
| Size | 22 | 25 | 168 |
| Primer 5 details |
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| Sequence | TGAGAGTGTCTCTTGACTTGACG | GCCTACTATAGCTCAATAGTGCTC | Start: 17 |
| Tm | 59.996 | 58.353 | End: 220 |
| Size | 23 | 24 | 204 |
