Primer Details for NODE_332707_length_381_cov_1.425325 |
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| (A)10tttgtagtagcaacttataataacgag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAACATTTTCTTTAAAAAGTTCACT | GCGTCGTCAGGTATATGCCT | Start: 240 |
| Tm | 57.786 | 59.686 | End: 374 |
| Size | 27 | 20 | 135 |
| Primer 2 details |
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| Sequence | TGCAACATTTTCTTTAAAAAGTTCACT | AGGCGTCGTCAGGTATATGC | Start: 240 |
| Tm | 57.786 | 59.686 | End: 376 |
| Size | 27 | 20 | 137 |
| Primer 3 details |
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| Sequence | AGTGCAACATTTTCTTTAAAAAGTTCA | GCGTCGTCAGGTATATGCCT | Start: 238 |
| Tm | 57.786 | 59.686 | End: 374 |
| Size | 27 | 20 | 137 |
| Primer 4 details |
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| Sequence | AGTGCAACATTTTCTTTAAAAAGTTCA | AGGCGTCGTCAGGTATATGC | Start: 238 |
| Tm | 57.786 | 59.686 | End: 376 |
| Size | 27 | 20 | 139 |
| Primer 5 details |
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| Sequence | GTGCAACATTTTCTTTAAAAAGTTCAC | GCGTCGTCAGGTATATGCCT | Start: 239 |
| Tm | 57.739 | 59.686 | End: 374 |
| Size | 27 | 20 | 136 |
