ssr_primer
Primer Details for NODE_323692_length_313_cov_1.083333 |
|---|
| (ACCTA)5accttaccttactttaccttact(TTACC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTTCCGTTACCTTACCT | AACGGAAGGCAAGGTAAAGT | Start: 23 |
| Tm | 58.352 | 57.349 | End: 273 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | ACTTTACCTTGCCTTCCGTT | AGGTAAGGTAACGGAAGGCA | Start: 14 |
| Tm | 57.349 | 58.352 | End: 282 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | CCTTCCGTTACCTTACCTTACCT | AAGGTAACGGAAGGCAAGGT | Start: 25 |
| Tm | 59.229 | 59.231 | End: 278 |
| Size | 23 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | ACCTTGCCTTCCGTTACCTT | AGGTAAGGTAAGGTAACGGAAGG | Start: 19 |
| Tm | 59.231 | 59.229 | End: 287 |
| Size | 20 | 23 | 269 |
| Primer 5 details |
|---|
| Sequence | TGCCTTCCGTTACCTTACCTT | AACGGAAGGCAAGGTAAAGT | Start: 23 |
| Tm | 59.02 | 57.349 | End: 273 |
| Size | 21 | 20 | 251 |
(ACCTA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCTTCCGTTACCTTACCT | AACGGAAGGCAAGGTAAAGT | Start: 23 |
| Tm | 58.352 | 57.349 | End: 273 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | ACTTTACCTTGCCTTCCGTT | AGGTAAGGTAACGGAAGGCA | Start: 14 |
| Tm | 57.349 | 58.352 | End: 282 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | CCTTCCGTTACCTTACCTTACCT | AAGGTAACGGAAGGCAAGGT | Start: 25 |
| Tm | 59.229 | 59.231 | End: 278 |
| Size | 23 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | ACCTTGCCTTCCGTTACCTT | AGGTAAGGTAAGGTAACGGAAGG | Start: 19 |
| Tm | 59.231 | 59.229 | End: 287 |
| Size | 20 | 23 | 269 |
| Primer 5 details |
|---|
| Sequence | TGCCTTCCGTTACCTTACCTT | AACGGAAGGCAAGGTAAAGT | Start: 23 |
| Tm | 59.02 | 57.349 | End: 273 |
| Size | 21 | 20 | 251 |
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