Primer Details for NODE_322754_length_234_cov_1.068323 |
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| (A)10gttacggcaagttacggcacattgtattacattgtattag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTACGGCAAGTTACGGCA | TCTTACACACTTTATAGGTGACGGT | Start: 10 |
| Tm | 59.966 | 59.522 | End: 109 |
| Size | 20 | 25 | 100 |
| Primer 2 details |
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| Sequence | AGTTACGGCAAGTTACGGCA | TCTTACACACTTTATAGGTGACGG | Start: 10 |
| Tm | 59.966 | 58.042 | End: 109 |
| Size | 20 | 24 | 100 |
| Primer 3 details |
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| Sequence | AGTTACGGCAAGTTACGGCA | TTCTTACACACTTTATAGGTGACGGT | Start: 10 |
| Tm | 59.966 | 60.015 | End: 110 |
| Size | 20 | 26 | 101 |
| Primer 4 details |
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| Sequence | GTTACGGCAAGTTACGGCAC | TTCTTACACACTTTATAGGTGACGGT | Start: 11 |
| Tm | 59.836 | 60.015 | End: 110 |
| Size | 20 | 26 | 100 |
| Primer 5 details |
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| Sequence | AGTTACGGCAAGTTACGGCA | TTCTTACACACTTTATAGGTGACGG | Start: 10 |
| Tm | 59.966 | 58.608 | End: 110 |
| Size | 20 | 25 | 101 |
