Primer Details for NODE_321979_length_250_cov_0.836158 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTATTATGGTTGCACAAAGTACT | ATTCGCGGTAGAACTCGGTC | Start: 128 |
| Tm | 59.064 | 59.9 | End: 227 |
| Size | 25 | 20 | 100 |
| Primer 2 details |
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| Sequence | AGTACGTATTATGGTTGCACAAAGT | ATTCGCGGTAGAACTCGGTC | Start: 125 |
| Tm | 59.064 | 59.9 | End: 227 |
| Size | 25 | 20 | 103 |
| Primer 3 details |
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| Sequence | AGGAAAAGTACGTATTATGGTTGCAC | ATTCGCGGTAGAACTCGGTC | Start: 119 |
| Tm | 59.907 | 59.9 | End: 227 |
| Size | 26 | 20 | 109 |
| Primer 4 details |
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| Sequence | GGAAAAGTACGTATTATGGTTGCACA | ATTCGCGGTAGAACTCGGTC | Start: 120 |
| Tm | 60.128 | 59.9 | End: 227 |
| Size | 26 | 20 | 108 |
| Primer 5 details |
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| Sequence | ACGTATTATGGTTGCACAAAGTAC | ATTCGCGGTAGAACTCGGTC | Start: 128 |
| Tm | 57.827 | 59.9 | End: 227 |
| Size | 24 | 20 | 100 |
