Primer Details for NODE_320452_length_354_cov_1.096085 | |||
|---|---|---|---|
| (A)10tta(T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTGTGCAAAATGGACTGACC | AGACTTTGACTTAAAAGGCCAGT | Start: 58 |
| Tm | 59.638 | 58.207 | End: 321 |
| Size | 22 | 23 | 264 |
| Primer 2 details | |||
| Sequence | ACTTGTGCAAAATGGACTGACC | CAAGACTTTGACTTAAAAGGCCAGT | Start: 58 |
| Tm | 59.638 | 59.932 | End: 323 |
| Size | 22 | 25 | 266 |
| Primer 3 details | |||
| Sequence | ACTTGTGCAAAATGGACTGACC | ACATTCGTAGTTAGCATTCCAGT | Start: 58 |
| Tm | 59.638 | 57.914 | End: 242 |
| Size | 22 | 23 | 185 |
| Primer 4 details | |||
| Sequence | ACTTGTGCAAAATGGACTGACC | TCAAGACTTTGACTTAAAAGGCCA | Start: 58 |
| Tm | 59.638 | 58.803 | End: 324 |
| Size | 22 | 24 | 267 |
| Primer 5 details | |||
| Sequence | ACTTGTGCAAAATGGACTGACC | AAGACTTTGACTTAAAAGGCCAGT | Start: 58 |
| Tm | 59.638 | 58.799 | End: 322 |
| Size | 22 | 24 | 265 |
