Primer Details for NODE_320343_length_287_cov_1.439252 | |||
|---|---|---|---|
| (TGT)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCACTCTGTTGTTGTTGT | TGAATCACCAATTAAAAGTCCACA | Start: 134 |
| Tm | 58.167 | 57.032 | End: 250 |
| Size | 20 | 24 | 117 |
| Primer 2 details | |||
| Sequence | TGGCACTCTGTTGTTGTTGT | ACATGAATCACCAATTAAAAGTCCACA | Start: 134 |
| Tm | 58.167 | 59.985 | End: 253 |
| Size | 20 | 27 | 120 |
| Primer 3 details | |||
| Sequence | GGCACTCTGTTGTTGTTGTTGT | TGAATCACCAATTAAAAGTCCACA | Start: 135 |
| Tm | 60.095 | 57.032 | End: 250 |
| Size | 22 | 24 | 116 |
| Primer 4 details | |||
| Sequence | TGGCACTCTGTTGTTGTTGTTG | TGAATCACCAATTAAAAGTCCACA | Start: 134 |
| Tm | 60.095 | 57.032 | End: 250 |
| Size | 22 | 24 | 117 |
| Primer 5 details | |||
| Sequence | GGCACTCTGTTGTTGTTGTTGT | ACATGAATCACCAATTAAAAGTCCACA | Start: 135 |
| Tm | 60.095 | 59.985 | End: 253 |
| Size | 22 | 27 | 119 |
