Primer Details for NODE_320104_length_265_cov_1.604167 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACACATCAATCATCACATGAAT | TGAACCAGCTAAACAAAAGGGT | Start: 97 |
| Tm | 57.231 | 58.362 | End: 252 |
| Size | 24 | 22 | 156 |
| Primer 2 details | |||
| Sequence | ACACACATCAATCATCACATGAAT | TTGAACCAGCTAAACAAAAGGGT | Start: 97 |
| Tm | 57.231 | 58.973 | End: 253 |
| Size | 24 | 23 | 157 |
| Primer 3 details | |||
| Sequence | ACACACATCAATCATCACATGAAT | ATTGAACCAGCTAAACAAAAGGGT | Start: 97 |
| Tm | 57.231 | 59.346 | End: 254 |
| Size | 24 | 24 | 158 |
| Primer 4 details | |||
| Sequence | ACACACATCAATCATCACATGAAT | TTGAACCAGCTAAACAAAAGGG | Start: 97 |
| Tm | 57.231 | 57.336 | End: 253 |
| Size | 24 | 22 | 157 |
| Primer 5 details | |||
| Sequence | ACACTTATACACACATCAATCATCACA | TGAACCAGCTAAACAAAAGGGT | Start: 89 |
| Tm | 59.176 | 58.362 | End: 252 |
| Size | 27 | 22 | 164 |
