Primer Details for NODE_319849_length_253_cov_1.711111 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGATTGAAGCTGTTGACATACA | ACGCATTCATAACACCTGGGT | Start: 77 |
| Tm | 58.692 | 59.996 | End: 216 |
| Size | 24 | 21 | 140 |
| Primer 2 details | |||
| Sequence | TTGACATACAACCAGGAATAGCT | ACGCATTCATAACACCTGGGT | Start: 91 |
| Tm | 57.564 | 59.996 | End: 216 |
| Size | 23 | 21 | 126 |
| Primer 3 details | |||
| Sequence | ACAACCAGGAATAGCTAATGGAA | ACGCATTCATAACACCTGGGT | Start: 98 |
| Tm | 57.549 | 59.996 | End: 216 |
| Size | 23 | 21 | 119 |
| Primer 4 details | |||
| Sequence | ACAGATTGAAGCTGTTGACATACAA | ACGCATTCATAACACCTGGGT | Start: 77 |
| Tm | 59.235 | 59.996 | End: 216 |
| Size | 25 | 21 | 140 |
| Primer 5 details | |||
| Sequence | ACAACCAGGAATAGCTAATGGAAA | ACGCATTCATAACACCTGGGT | Start: 98 |
| Tm | 58.173 | 59.996 | End: 216 |
| Size | 24 | 21 | 119 |
