Primer Details for NODE_319478_length_282_cov_1.473684 | |||
|---|---|---|---|
| (A)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCTTGCTGCTATTAATGATGG | AGCATATCCAGTGTTTGTCCCA | Start: 32 |
| Tm | 59.177 | 59.691 | End: 275 |
| Size | 24 | 22 | 244 |
| Primer 2 details | |||
| Sequence | GGTCTTGCTGCTATTAATGATGGA | AGCATATCCAGTGTTTGTCCCA | Start: 33 |
| Tm | 58.934 | 59.691 | End: 275 |
| Size | 24 | 22 | 243 |
| Primer 3 details | |||
| Sequence | TGGTCTTGCTGCTATTAATGATGG | GCATATCCAGTGTTTGTCCCA | Start: 32 |
| Tm | 59.177 | 58.278 | End: 274 |
| Size | 24 | 21 | 243 |
| Primer 4 details | |||
| Sequence | TTGGTCTTGCTGCTATTAATGATGG | AGCATATCCAGTGTTTGTCCCA | Start: 31 |
| Tm | 59.7 | 59.691 | End: 275 |
| Size | 25 | 22 | 245 |
| Primer 5 details | |||
| Sequence | GGTCTTGCTGCTATTAATGATGG | AGCATATCCAGTGTTTGTCCCA | Start: 33 |
| Tm | 57.632 | 59.691 | End: 275 |
| Size | 23 | 22 | 243 |
