Primer Details for NODE_318778_length_280_cov_1.487923 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAATGGAAAAAGTAGGCACATTT | AGTGTGATAACTGACAACTTACCT | Start: 24 |
| Tm | 57.582 | 57.579 | End: 255 |
| Size | 24 | 24 | 232 |
| Primer 2 details | |||
| Sequence | TTGAATGGAAAAAGTAGGCACATT | AGTGTGATAACTGACAACTTACCT | Start: 23 |
| Tm | 57.582 | 57.579 | End: 255 |
| Size | 24 | 24 | 233 |
| Primer 3 details | |||
| Sequence | TGAATGGAAAAAGTAGGCACATTT | AGAGTGTTCTAGTGTGATAACTGACA | Start: 24 |
| Tm | 57.582 | 59.51 | End: 265 |
| Size | 24 | 26 | 242 |
| Primer 4 details | |||
| Sequence | TTGAATGGAAAAAGTAGGCACATT | AGAGTGTTCTAGTGTGATAACTGACA | Start: 23 |
| Tm | 57.582 | 59.51 | End: 265 |
| Size | 24 | 26 | 243 |
| Primer 5 details | |||
| Sequence | ATTGAATGGAAAAAGTAGGCACAT | AGTGTGATAACTGACAACTTACCT | Start: 22 |
| Tm | 57.39 | 57.579 | End: 255 |
| Size | 24 | 24 | 234 |
