Primer Details for NODE_318742_length_261_cov_1.638298 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATCTGTACCTTGGCACA | TGCTTTCCACAAATGATTCATGT | Start: 118 |
| Tm | 57.051 | 57.528 | End: 259 |
| Size | 20 | 23 | 142 |
| Primer 2 details | |||
| Sequence | ACAATCTGTACCTTGGCACA | CTGCTTTCCACAAATGATTCATGT | Start: 118 |
| Tm | 57.051 | 58.524 | End: 260 |
| Size | 20 | 24 | 143 |
| Primer 3 details | |||
| Sequence | ACAATCTGTACCTTGGCACA | TGCTTTCCACAAATGATTCATGTA | Start: 118 |
| Tm | 57.051 | 57.413 | End: 259 |
| Size | 20 | 24 | 142 |
| Primer 4 details | |||
| Sequence | ACAATCTGTACCTTGGCACA | CTGCTTTCCACAAATGATTCATGTA | Start: 118 |
| Tm | 57.051 | 58.377 | End: 260 |
| Size | 20 | 25 | 143 |
| Primer 5 details | |||
| Sequence | TTACAATCTGTACCTTGGCACA | TGCTTTCCACAAATGATTCATGT | Start: 116 |
| Tm | 57.641 | 57.528 | End: 259 |
| Size | 22 | 23 | 144 |
