Primer Details for NODE_318682_length_282_cov_1.473684 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAACGGCGTTAATTTAAGTGA | TTTTTGCGCATTGGTAAGTGA | Start: 60 |
| Tm | 57.689 | 57.283 | End: 178 |
| Size | 22 | 21 | 119 |
| Primer 2 details | |||
| Sequence | AGTAGTTTAGTCTTTCCGTTTTTGT | TAACATGTGTAGCGCCCCTC | Start: 28 |
| Tm | 57.145 | 59.823 | End: 130 |
| Size | 25 | 20 | 103 |
| Primer 3 details | |||
| Sequence | CGAACGGCGTTAATTTAAGTGA | TTTGCGCATTGGTAAGTGATT | Start: 60 |
| Tm | 57.689 | 57.067 | End: 176 |
| Size | 22 | 21 | 117 |
| Primer 4 details | |||
| Sequence | CGAACGGCGTTAATTTAAGTGA | TTTTGCGCATTGGTAAGTGAT | Start: 60 |
| Tm | 57.689 | 57.067 | End: 177 |
| Size | 22 | 21 | 118 |
| Primer 5 details | |||
| Sequence | AGTAGTTTAGTCTTTCCGTTTTTGTT | TAACATGTGTAGCGCCCCTC | Start: 28 |
| Tm | 57.727 | 59.823 | End: 130 |
| Size | 26 | 20 | 103 |
