Primer Details for NODE_318099_length_282_cov_1.473684 |
|---|
| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTCTCAAGTTGATTGACAAGT | GTCTTGATGACGAAACAGATTACT | Start: 165 |
| Tm | 58.545 | 57.047 | End: 276 |
| Size | 23 | 24 | 112 |
| Primer 2 details |
|---|
| Sequence | GCTCTCAAGTTGATTGACAAGTCA | GTCTTGATGACGAAACAGATTACT | Start: 166 |
| Tm | 59.487 | 57.047 | End: 276 |
| Size | 24 | 24 | 111 |
| Primer 3 details |
|---|
| Sequence | TGCTCTCAAGTTGATTGACAAGTC | GTCTTGATGACGAAACAGATTACT | Start: 165 |
| Tm | 59.487 | 57.047 | End: 276 |
| Size | 24 | 24 | 112 |
| Primer 4 details |
|---|
| Sequence | TGCTCTCAAGTTGATTGACAAGT | GTCTTGATGACGAAACAGATTACTTGA | Start: 165 |
| Tm | 58.545 | 59.884 | End: 276 |
| Size | 23 | 27 | 112 |
| Primer 5 details |
|---|
| Sequence | TGCTCTCAAGTTGATTGACAAGT | TCTTGATGACGAAACAGATTACTTGA | Start: 165 |
| Tm | 58.545 | 58.848 | End: 275 |
| Size | 23 | 26 | 111 |
(A)12taattgctctcaagttgattgacaagtcaacagaattgattgtaaatttattat(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTCTCAAGTTGATTGACAAGT | GTCTTGATGACGAAACAGATTACT | Start: 165 |
| Tm | 58.545 | 57.047 | End: 276 |
| Size | 23 | 24 | 112 |
| Primer 2 details |
|---|
| Sequence | GCTCTCAAGTTGATTGACAAGTCA | GTCTTGATGACGAAACAGATTACT | Start: 166 |
| Tm | 59.487 | 57.047 | End: 276 |
| Size | 24 | 24 | 111 |
| Primer 3 details |
|---|
| Sequence | TGCTCTCAAGTTGATTGACAAGTC | GTCTTGATGACGAAACAGATTACT | Start: 165 |
| Tm | 59.487 | 57.047 | End: 276 |
| Size | 24 | 24 | 112 |
| Primer 4 details |
|---|
| Sequence | TGCTCTCAAGTTGATTGACAAGT | GTCTTGATGACGAAACAGATTACTTGA | Start: 165 |
| Tm | 58.545 | 59.884 | End: 276 |
| Size | 23 | 27 | 112 |
| Primer 5 details |
|---|
| Sequence | TGCTCTCAAGTTGATTGACAAGT | TCTTGATGACGAAACAGATTACTTGA | Start: 165 |
| Tm | 58.545 | 58.848 | End: 275 |
| Size | 23 | 26 | 111 |
