Primer Details for NODE_317949_length_253_cov_1.711111 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCGCTCACAGTGTTTTCC | TCAATGCATTGATACAGAACACAGT | Start: 81 |
| Tm | 59.331 | 59.293 | End: 180 |
| Size | 21 | 25 | 100 |
| Primer 2 details |
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| Sequence | TTGTCGCTCACAGTGTTTTCC | TCAATGCATTGATACAGAACACAG | Start: 81 |
| Tm | 59.331 | 57.814 | End: 180 |
| Size | 21 | 24 | 100 |
| Primer 3 details |
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| Sequence | TTGTCGCTCACAGTGTTTTCC | TCAATGCATTGATACAGAACACAGTT | Start: 81 |
| Tm | 59.331 | 59.793 | End: 180 |
| Size | 21 | 26 | 100 |
| Primer 4 details |
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| Sequence | ATTGTCGCTCACAGTGTTTTCC | TCAATGCATTGATACAGAACACAGT | Start: 80 |
| Tm | 59.709 | 59.293 | End: 180 |
| Size | 22 | 25 | 101 |
| Primer 5 details |
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| Sequence | ATTGTCGCTCACAGTGTTTTCC | CAATGCATTGATACAGAACACAGT | Start: 80 |
| Tm | 59.709 | 58.054 | End: 179 |
| Size | 22 | 24 | 100 |
