Primer Details for NODE_317073_length_290_cov_1.419355 |
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| (A)13gtttaattagagtataaaccgtgagtttgtgacatcgtgatgtgttttaataaagtccaaaa(AAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTGAGTTTGTGACATCGTG | AGCTGATAAGAAATTTGCTTCCTGA | Start: 71 |
| Tm | 59.808 | 59.052 | End: 220 |
| Size | 21 | 25 | 150 |
| Primer 2 details |
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| Sequence | ACCGTGAGTTTGTGACATCGT | AGCTGATAAGAAATTTGCTTCCTGA | Start: 70 |
| Tm | 60.202 | 59.052 | End: 220 |
| Size | 21 | 25 | 151 |
| Primer 3 details |
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| Sequence | CCGTGAGTTTGTGACATCGTG | GCTGATAAGAAATTTGCTTCCTGA | Start: 71 |
| Tm | 59.808 | 57.8 | End: 219 |
| Size | 21 | 24 | 149 |
| Primer 4 details |
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| Sequence | CCGTGAGTTTGTGACATCGTG | AGCTGATAAGAAATTTGCTTCCTG | Start: 71 |
| Tm | 59.808 | 57.795 | End: 220 |
| Size | 21 | 24 | 150 |
| Primer 5 details |
|---|
| Sequence | ACCGTGAGTTTGTGACATCGT | GCTGATAAGAAATTTGCTTCCTGA | Start: 70 |
| Tm | 60.202 | 57.8 | End: 219 |
| Size | 21 | 24 | 150 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTGAGTTTGTGACATCGTG | AGCTGATAAGAAATTTGCTTCCTGA | Start: 71 |
| Tm | 59.808 | 59.052 | End: 220 |
| Size | 21 | 25 | 150 |
| Primer 2 details |
|---|
| Sequence | ACCGTGAGTTTGTGACATCGT | AGCTGATAAGAAATTTGCTTCCTGA | Start: 70 |
| Tm | 60.202 | 59.052 | End: 220 |
| Size | 21 | 25 | 151 |
| Primer 3 details |
|---|
| Sequence | CCGTGAGTTTGTGACATCGTG | GCTGATAAGAAATTTGCTTCCTGA | Start: 71 |
| Tm | 59.808 | 57.8 | End: 219 |
| Size | 21 | 24 | 149 |
| Primer 4 details |
|---|
| Sequence | CCGTGAGTTTGTGACATCGTG | AGCTGATAAGAAATTTGCTTCCTG | Start: 71 |
| Tm | 59.808 | 57.795 | End: 220 |
| Size | 21 | 24 | 150 |
| Primer 5 details |
|---|
| Sequence | ACCGTGAGTTTGTGACATCGT | GCTGATAAGAAATTTGCTTCCTGA | Start: 70 |
| Tm | 60.202 | 57.8 | End: 219 |
| Size | 21 | 24 | 150 |
