Primer Details for NODE_316355_length_251_cov_0.865169 |
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| (T)23gtcttctttgttttgttgaaagtgatgatgatttttatttttttattaaatatgtat(A)11gtaaaata(T)34gtcttcttttgtcttc(T)24gtcttcttgcatcctttcatttcctccc(T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTTCTTTGTTTTGTTGAAAGTGA | AGGGAGGAAATGAAAGGATGCA | Start: 22 |
| Tm | 58.57 | 59.687 | End: 201 |
| Size | 26 | 22 | 180 |
| Primer 2 details |
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| Sequence | TGTCTTCTTTGTTTTGTTGAAAGTG | AGGGAGGAAATGAAAGGATGCA | Start: 22 |
| Tm | 57.364 | 59.687 | End: 201 |
| Size | 25 | 22 | 180 |
| Primer 3 details |
|---|
| Sequence | TGTCTTCTTTGTTTTGTTGAAAGTGA | GGGAGGAAATGAAAGGATGCA | Start: 22 |
| Tm | 58.57 | 58.261 | End: 200 |
| Size | 26 | 21 | 179 |
| Primer 4 details |
|---|
| Sequence | GTCTTCTTTGTTTTGTTGAAAGTGA | AGGGAGGAAATGAAAGGATGCA | Start: 23 |
| Tm | 57.133 | 59.687 | End: 201 |
| Size | 25 | 22 | 179 |
| Primer 5 details |
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| Sequence | TTGTCTTCTTTGTTTTGTTGAAAGTGA | AGGGAGGAAATGAAAGGATGCA | Start: 21 |
| Tm | 59.077 | 59.687 | End: 201 |
| Size | 27 | 22 | 181 |
