Primer Details for NODE_316319_length_231_cov_1.183544 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGATGCTTGCTTCTGTTTTACA | AGCTAAAACTCAAAAAGCACACA | Start: 41 |
| Tm | 59.662 | 57.677 | End: 178 |
| Size | 24 | 23 | 138 |
| Primer 2 details | |||
| Sequence | TGTGATGCTTGCTTCTGTTTTACA | GCTAAAACTCAAAAAGCACACAAGT | Start: 41 |
| Tm | 59.662 | 59.423 | End: 177 |
| Size | 24 | 25 | 137 |
| Primer 3 details | |||
| Sequence | ATGTGATGCTTGCTTCTGTTTT | AGCTAAAACTCAAAAAGCACACA | Start: 40 |
| Tm | 57.404 | 57.677 | End: 178 |
| Size | 22 | 23 | 139 |
| Primer 4 details | |||
| Sequence | AATGTGATGCTTGCTTCTGTTT | AGCTAAAACTCAAAAAGCACACA | Start: 39 |
| Tm | 57.404 | 57.677 | End: 178 |
| Size | 22 | 23 | 140 |
| Primer 5 details | |||
| Sequence | TGTGATGCTTGCTTCTGTTTTACA | AGCTAAAACTCAAAAAGCACACAA | Start: 41 |
| Tm | 59.662 | 58.281 | End: 178 |
| Size | 24 | 24 | 138 |
