Primer Details for NODE_316116_length_231_cov_0.974684 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCTTCTGGATTTGATGTAACTTGA | CAGACACACGAAAACGCGAG | Start: 42 |
| Tm | 57.31 | 60.112 | End: 213 |
| Size | 26 | 20 | 172 |
| Primer 2 details |
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| Sequence | TTTCTTCTGGATTTGATGTAACTTGA | GACACACGAAAACGCGAGAG | Start: 42 |
| Tm | 57.31 | 59.841 | End: 211 |
| Size | 26 | 20 | 170 |
| Primer 3 details |
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| Sequence | TTTCTTCTGGATTTGATGTAACTTGA | ACACACGAAAACGCGAGAGA | Start: 42 |
| Tm | 57.31 | 60.249 | End: 210 |
| Size | 26 | 20 | 169 |
| Primer 4 details |
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| Sequence | TTTCTTCTGGATTTGATGTAACTTGA | AGACACACGAAAACGCGAGA | Start: 42 |
| Tm | 57.31 | 60.249 | End: 212 |
| Size | 26 | 20 | 171 |
| Primer 5 details |
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| Sequence | TCTTCTGGATTTGATGTAACTTGATT | CAGACACACGAAAACGCGAG | Start: 44 |
| Tm | 57.131 | 60.112 | End: 213 |
| Size | 26 | 20 | 170 |
