Primer Details for NODE_314337_length_230_cov_0.980892 | |||
|---|---|---|---|
| (ATG)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCCATTTTCTTTGTTATCACGT | ACGTGCAGCATCAACATCTG | Start: 26 |
| Tm | 59.72 | 59.199 | End: 148 |
| Size | 24 | 20 | 123 |
| Primer 2 details | |||
| Sequence | TGGCCATTTTCTTTGTTATCACGT | CGTGCAGCATCAACATCTGA | Start: 26 |
| Tm | 59.72 | 58.919 | End: 147 |
| Size | 24 | 20 | 122 |
| Primer 3 details | |||
| Sequence | TGGCCATTTTCTTTGTTATCACG | ACGTGCAGCATCAACATCTG | Start: 26 |
| Tm | 58.196 | 59.199 | End: 148 |
| Size | 23 | 20 | 123 |
| Primer 4 details | |||
| Sequence | GGCCATTTTCTTTGTTATCACGT | ACGTGCAGCATCAACATCTG | Start: 27 |
| Tm | 58.195 | 59.199 | End: 148 |
| Size | 23 | 20 | 122 |
| Primer 5 details | |||
| Sequence | ATGGCCATTTTCTTTGTTATCACGT | ACGTGCAGCATCAACATCTG | Start: 25 |
| Tm | 60.047 | 59.199 | End: 148 |
| Size | 25 | 20 | 124 |
