Primer Details for NODE_311685_length_230_cov_0.757962 |
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| (T)23gttggagtttttattatagggggagaa(T)13gagttttgtaatttattttttattatttttatttttgtttttttttg(T)29gtttccacctgccatattcccta(T)39: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGGAGTTTTTATTATAGGGGGAGA | AGGGAATATGGCAGGTGGAA | Start: 34 |
| Tm | 59.815 | 58.393 | End: 172 |
| Size | 27 | 20 | 139 |
| Primer 2 details |
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| Sequence | TGTTGGAGTTTTTATTATAGGGGGA | AGGGAATATGGCAGGTGGAA | Start: 34 |
| Tm | 57.713 | 58.393 | End: 172 |
| Size | 25 | 20 | 139 |
| Primer 3 details |
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| Sequence | TGTTGGAGTTTTTATTATAGGGGGAG | AGGGAATATGGCAGGTGGAA | Start: 34 |
| Tm | 58.649 | 58.393 | End: 172 |
| Size | 26 | 20 | 139 |
| Primer 4 details |
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| Sequence | TGTTGGAGTTTTTATTATAGGGGGAGA | AGGGAATATGGCAGGTGGAAA | Start: 34 |
| Tm | 59.815 | 59.071 | End: 172 |
| Size | 27 | 21 | 139 |
| Primer 5 details |
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| Sequence | GTTGGAGTTTTTATTATAGGGGGAGA | AGGGAATATGGCAGGTGGAA | Start: 35 |
| Tm | 58.42 | 58.393 | End: 172 |
| Size | 26 | 20 | 138 |
