Primer Details for NODE_311563_length_268_cov_1.066667 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATCTGGTGTGTTCTAGTGTGT | GGTATGTTGTGGTTTTCTTTTTCACA | Start: 104 |
| Tm | 59.359 | 59.186 | End: 205 |
| Size | 23 | 26 | 102 |
| Primer 2 details |
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| Sequence | TGTGAAAAAGAAAACCACAACATACC | AGAACACACTAGAACACACCAGA | Start: 30 |
| Tm | 59.186 | 59.295 | End: 129 |
| Size | 26 | 23 | 100 |
| Primer 3 details |
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| Sequence | TCAATGTGAAAAAGAAAACCACAACA | ACACACTAGAACACACCAGATCA | Start: 26 |
| Tm | 59.069 | 59.359 | End: 126 |
| Size | 26 | 23 | 101 |
| Primer 4 details |
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| Sequence | TCAATGTGAAAAAGAAAACCACAACA | AGAACACACTAGAACACACCAGA | Start: 26 |
| Tm | 59.069 | 59.295 | End: 129 |
| Size | 26 | 23 | 104 |
| Primer 5 details |
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| Sequence | TGATCTGGTGTGTTCTAGTGTGT | AGGTATGTTGTGGTTTTCTTTTTCAC | Start: 104 |
| Tm | 59.359 | 58.961 | End: 206 |
| Size | 23 | 26 | 103 |
