Primer Details for NODE_311340_length_238_cov_1.060606 | |||
|---|---|---|---|
| (T)11(A)10gataaatccggcatgaaactaaaaaaaaat(A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCAGACTGAATCCCCGCG | TGAATCGACAGACAAGAAGATGT | Start: 3 |
| Tm | 60.179 | 57.861 | End: 118 |
| Size | 20 | 23 | 116 |
| Primer 2 details | |||
| Sequence | AATCAGACTGAATCCCCGCG | AAGATGTCTCCAAAAGTTAACCAGC | Start: 3 |
| Tm | 60.179 | 59.758 | End: 102 |
| Size | 20 | 25 | 100 |
| Primer 3 details | |||
| Sequence | AATCAGACTGAATCCCCGCG | TTGAATCGACAGACAAGAAGATGT | Start: 3 |
| Tm | 60.179 | 58.458 | End: 119 |
| Size | 20 | 24 | 117 |
| Primer 4 details | |||
| Sequence | CGGAATCAGACTGAATCCCCG | ATGTCTCCAAAAGTTAACCAGCTG | Start: 0 |
| Tm | 60.539 | 59.48 | End: 99 |
| Size | 21 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AATCAGACTGAATCCCCGCG | CGTTCTGTGAAATTTTGAATCGACA | Start: 3 |
| Tm | 60.179 | 59.093 | End: 133 |
| Size | 20 | 25 | 131 |
