Primer Details for NODE_310832_length_229_cov_1.160256 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACAAACAAAGCAATTTTTCAGT | AGCTTTTTGACTTCTTAAGCAGCA | Start: 53 |
| Tm | 59.063 | 59.661 | End: 180 |
| Size | 25 | 24 | 128 |
| Primer 2 details |
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| Sequence | TGGACAAACAAAGCAATTTTTCAGT | GCTTTTTGACTTCTTAAGCAGCA | Start: 53 |
| Tm | 59.063 | 58.389 | End: 179 |
| Size | 25 | 23 | 127 |
| Primer 3 details |
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| Sequence | TGGACAAACAAAGCAATTTTTCAGT | GCTTTTTGACTTCTTAAGCAGCAG | Start: 53 |
| Tm | 59.063 | 59.321 | End: 179 |
| Size | 25 | 24 | 127 |
| Primer 4 details |
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| Sequence | TGGACAAACAAAGCAATTTTTCAG | AGCTTTTTGACTTCTTAAGCAGCA | Start: 53 |
| Tm | 57.58 | 59.661 | End: 180 |
| Size | 24 | 24 | 128 |
| Primer 5 details |
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| Sequence | GGACAAACAAAGCAATTTTTCAGT | AGCTTTTTGACTTCTTAAGCAGCA | Start: 54 |
| Tm | 57.579 | 59.661 | End: 180 |
| Size | 24 | 24 | 127 |
