Primer Details for NODE_309877_length_248_cov_1.914286 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGAGAAAAGTGAACTGTTTCA | CAGTTCAACTTTGCACTGTTTGT | Start: 56 |
| Tm | 58.747 | 58.763 | End: 165 |
| Size | 24 | 23 | 110 |
| Primer 2 details | |||
| Sequence | ACAGGAGAAAAGTGAACTGTTTCA | AGTTCAACTTTGCACTGTTTGT | Start: 56 |
| Tm | 58.747 | 57.48 | End: 164 |
| Size | 24 | 22 | 109 |
| Primer 3 details | |||
| Sequence | ACAGGAGAAAAGTGAACTGTTTCAA | CAGTTCAACTTTGCACTGTTTGT | Start: 56 |
| Tm | 59.29 | 58.763 | End: 165 |
| Size | 25 | 23 | 110 |
| Primer 4 details | |||
| Sequence | AACAGGAGAAAAGTGAACTGTTTCA | CAGTTCAACTTTGCACTGTTTGT | Start: 55 |
| Tm | 59.29 | 58.763 | End: 165 |
| Size | 25 | 23 | 111 |
| Primer 5 details | |||
| Sequence | CAGGAGAAAAGTGAACTGTTTCA | CAGTTCAACTTTGCACTGTTTGT | Start: 57 |
| Tm | 57.182 | 58.763 | End: 165 |
| Size | 23 | 23 | 109 |
