Primer Details for NODE_306464_length_250_cov_0.864407 |
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| (T)23aggggattttgaccgtaattatgaacacttgattatctttcgtttctcctttcccgttgatgcatcaggcacttctc(T)33aggggattttagggga(T)24aggggaataattttg(T)13attttttaa(T)13attatatataat(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTATCTTTCGTTTCTCCTTTCCCG | AAAAATCCCCTAAAATCCCCTAAAAA | Start: 55 |
| Tm | 59.415 | 57.473 | End: 154 |
| Size | 25 | 26 | 100 |
| Primer 2 details |
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| Sequence | AGGGGATTTTGACCGTAATTATGA | AAAAATCCCCTAAAATCCCCTAAAAA | Start: 24 |
| Tm | 57.991 | 57.473 | End: 154 |
| Size | 24 | 26 | 131 |
| Primer 3 details |
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| Sequence | GGGGATTTTGACCGTAATTATGAACA | AAAAATCCCCTAAAATCCCCTAAAAA | Start: 25 |
| Tm | 59.904 | 57.473 | End: 154 |
| Size | 26 | 26 | 130 |
| Primer 4 details |
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| Sequence | GATTATCTTTCGTTTCTCCTTTCCCG | AAAAATCCCCTAAAATCCCCTAAAAA | Start: 54 |
| Tm | 60.238 | 57.473 | End: 154 |
| Size | 26 | 26 | 101 |
| Primer 5 details |
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| Sequence | AGGGGATTTTGACCGTAATTATGAAC | AAAAATCCCCTAAAATCCCCTAAAAA | Start: 24 |
| Tm | 59.68 | 57.473 | End: 154 |
| Size | 26 | 26 | 131 |
