Primer Details for NODE_306320_length_229_cov_0.987179 | |||
|---|---|---|---|
| (TGT)5cattattgttgg(TGC)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGTTGGTGCTGCTGCTG | ACATACATCAACAACACTTGCTGT | Start: 43 |
| Tm | 59.117 | 59.42 | End: 193 |
| Size | 20 | 24 | 151 |
| Primer 2 details | |||
| Sequence | TATTGTTGGTGCTGCTGCTG | ACATCAACAACACTTGCTGTATCA | Start: 43 |
| Tm | 59.117 | 59.179 | End: 189 |
| Size | 20 | 24 | 147 |
| Primer 3 details | |||
| Sequence | TATTGTTGGTGCTGCTGCTG | ACAACACTTGCTGTATCAAATCCA | Start: 43 |
| Tm | 59.117 | 59.173 | End: 183 |
| Size | 20 | 24 | 141 |
| Primer 4 details | |||
| Sequence | TTATTGTTGGTGCTGCTGCTG | ACATACATCAACAACACTTGCTGT | Start: 42 |
| Tm | 59.73 | 59.42 | End: 193 |
| Size | 21 | 24 | 152 |
| Primer 5 details | |||
| Sequence | TATTGTTGGTGCTGCTGCTG | TGCTGTATCAAATCCAAAACAAGCT | Start: 43 |
| Tm | 59.117 | 59.989 | End: 175 |
| Size | 20 | 25 | 133 |
