ssr_primer
Primer Details for NODE_305731_length_250_cov_0.870056 |
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| (T)22ctttcccccgcgggccccctttttc(T)13aaagtttttattttattggaaaaccaaaccttg(T)39ctttccccttctttcccc(T)22ctttcccctcttcatcaccttttcc(T)12cttttttc(T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGCCCCCTTTTTCTTTTT | AGGGGAAAGAAGGGGAAAGA | Start: 32 |
| Tm | 59.238 | 57.85 | End: 150 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
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| Sequence | CGGGCCCCCTTTTTCTTTTT | GGAAAAGGTGATGAAGAGGGGA | Start: 32 |
| Tm | 59.238 | 59.691 | End: 196 |
| Size | 20 | 22 | 165 |
| Primer 3 details |
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| Sequence | CGGGCCCCCTTTTTCTTTTT | AGGAAAAGGTGATGAAGAGGGG | Start: 32 |
| Tm | 59.238 | 59.69 | End: 197 |
| Size | 20 | 22 | 166 |
| Primer 4 details |
|---|
| Sequence | CGGGCCCCCTTTTTCTTTTT | AGGGGAAAGAAGGGGAAAGAA | Start: 32 |
| Tm | 59.238 | 58.561 | End: 150 |
| Size | 20 | 21 | 119 |
| Primer 5 details |
|---|
| Sequence | CGGGCCCCCTTTTTCTTTTT | AAGGGGAAAGAAGGGGAAAGA | Start: 32 |
| Tm | 59.238 | 58.561 | End: 151 |
| Size | 20 | 21 | 120 |
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