Primer Details for NODE_303144_length_219_cov_2.109589 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACAGGTATTAGTGAAAAAGTTTCA | ATTTTTATTGTCAAACTAACGTCAACA | Start: 67 |
| Tm | 57.486 | 57.21 | End: 166 |
| Size | 26 | 27 | 100 |
| Primer 2 details |
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| Sequence | AACACAGGTATTAGTGAAAAAGTTTCA | TTTTTATTGTCAAACTAACGTCAACAT | Start: 66 |
| Tm | 58.037 | 57.21 | End: 165 |
| Size | 27 | 27 | 100 |
| Primer 3 details |
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| Sequence | AACACAGGTATTAGTGAAAAAGTTTCA | ATTTTTATTGTCAAACTAACGTCAACA | Start: 66 |
| Tm | 58.037 | 57.21 | End: 166 |
| Size | 27 | 27 | 101 |
| Primer 4 details |
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| Sequence | ACACAGGTATTAGTGAAAAAGTTTCAT | ATTTTTATTGTCAAACTAACGTCAACA | Start: 67 |
| Tm | 57.868 | 57.21 | End: 166 |
| Size | 27 | 27 | 100 |
| Primer 5 details |
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| Sequence | 166 | 166 | Start: 166 |
| Tm | 166 | 166 | End: 166 |
| Size | 166 | 166 | 166 |
