Primer Details for NODE_300607_length_257_cov_0.956522 | |||
|---|---|---|---|
| (T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCCAAATCATTCACCACCT | AGATGGTCTGGTCAATAAATGTCA | Start: 128 |
| Tm | 57.476 | 57.934 | End: 256 |
| Size | 21 | 24 | 129 |
| Primer 2 details | |||
| Sequence | GTCCAAATCATTCACCACCTTCA | AGATGGTCTGGTCAATAAATGTCA | Start: 129 |
| Tm | 59.174 | 57.934 | End: 256 |
| Size | 23 | 24 | 128 |
| Primer 3 details | |||
| Sequence | TGTCCAAATCATTCACCACCTTC | AGATGGTCTGGTCAATAAATGTCA | Start: 128 |
| Tm | 59.174 | 57.934 | End: 256 |
| Size | 23 | 24 | 129 |
| Primer 4 details | |||
| Sequence | TGTCCAAATCATTCACCACCTT | AGATGGTCTGGTCAATAAATGTCA | Start: 128 |
| Tm | 58.157 | 57.934 | End: 256 |
| Size | 22 | 24 | 129 |
| Primer 5 details | |||
| Sequence | TGTCCAAATCATTCACCACCT | AGATGGTCTGGTCAATAAATGTCAA | Start: 128 |
| Tm | 57.476 | 58.516 | End: 256 |
| Size | 21 | 25 | 129 |
