ssr_primer
Primer Details for NODE_2990_length_4528_cov_21.808305 |
|---|
| (CAG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCGGACTCCTGTGATCGA | GGGTGTGCTTGAAGTGCTTG | Start: 360 |
| Tm | 60.035 | 59.969 | End: 585 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CAAGCACTTCAAGCACACCC | AGCATTGCAAGAGCTCCACT | Start: 566 |
| Tm | 59.969 | 59.962 | End: 679 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | TGGACTCGGACTCCTGTGAT | GGGTGTGCTTGAAGTGCTTG | Start: 357 |
| Tm | 59.96 | 59.969 | End: 585 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | AATTCAACAGCAGCAGCAGC | AGCTTGACCGCATGAAGTCA | Start: 1563 |
| Tm | 60.039 | 59.965 | End: 1678 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | TGACTTCATGCGGTCAAGCT | TGCTTTCCATAGGGCTTGCA | Start: 1659 |
| Tm | 59.965 | 59.961 | End: 1895 |
| Size | 20 | 20 | 237 |
(TTG)6tattgccatgttccttttttgtgttttagagtaatttttggaagcatttttaaa(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCGGACTCCTGTGATCGA | GGGTGTGCTTGAAGTGCTTG | Start: 360 |
| Tm | 60.035 | 59.969 | End: 585 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CAAGCACTTCAAGCACACCC | AGCATTGCAAGAGCTCCACT | Start: 566 |
| Tm | 59.969 | 59.962 | End: 679 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | TGGACTCGGACTCCTGTGAT | GGGTGTGCTTGAAGTGCTTG | Start: 357 |
| Tm | 59.96 | 59.969 | End: 585 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | AATTCAACAGCAGCAGCAGC | AGCTTGACCGCATGAAGTCA | Start: 1563 |
| Tm | 60.039 | 59.965 | End: 1678 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | TGACTTCATGCGGTCAAGCT | TGCTTTCCATAGGGCTTGCA | Start: 1659 |
| Tm | 59.965 | 59.961 | End: 1895 |
| Size | 20 | 20 | 237 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCGGACTCCTGTGATCGA | GGGTGTGCTTGAAGTGCTTG | Start: 360 |
| Tm | 60.035 | 59.969 | End: 585 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CAAGCACTTCAAGCACACCC | AGCATTGCAAGAGCTCCACT | Start: 566 |
| Tm | 59.969 | 59.962 | End: 679 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | TGGACTCGGACTCCTGTGAT | GGGTGTGCTTGAAGTGCTTG | Start: 357 |
| Tm | 59.96 | 59.969 | End: 585 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | AATTCAACAGCAGCAGCAGC | AGCTTGACCGCATGAAGTCA | Start: 1563 |
| Tm | 60.039 | 59.965 | End: 1678 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | TGACTTCATGCGGTCAAGCT | TGCTTTCCATAGGGCTTGCA | Start: 1659 |
| Tm | 59.965 | 59.961 | End: 1895 |
| Size | 20 | 20 | 237 |
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