Primer Details for NODE_295225_length_247_cov_0.862069 |
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| (G)24agggaagagtgtggtgggtgttgtggagtgtgtgggaagacaaacccacaaaacaaaaaaaggaaaggagagtg(A)33gaag(A)12gataaagaaagcgtaaagacaaattgaaag(A)10ggagatatagaaattgaaagaaattgaaag(A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGTGTGGTGGGTGTTGTG | TCTTTCAATTTGTCTTTACGCTTTCT | Start: 29 |
| Tm | 60.107 | 58.297 | End: 177 |
| Size | 20 | 26 | 149 |
| Primer 2 details |
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| Sequence | GTGTTGTGGAGTGTGTGGGA | TCTTTCAATTTGTCTTTACGCTTTCT | Start: 41 |
| Tm | 60.107 | 58.297 | End: 177 |
| Size | 20 | 26 | 137 |
| Primer 3 details |
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| Sequence | GGAAGAGTGTGGTGGGTGTT | TCTTTCAATTTGTCTTTACGCTTTCT | Start: 26 |
| Tm | 59.817 | 58.297 | End: 177 |
| Size | 20 | 26 | 152 |
| Primer 4 details |
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| Sequence | AGAGTGTGGTGGGTGTTGTG | TCTTTCAATTTGTCTTTACGCTTTC | Start: 29 |
| Tm | 60.107 | 57.092 | End: 177 |
| Size | 20 | 25 | 149 |
| Primer 5 details |
|---|
| Sequence | GTGTTGTGGAGTGTGTGGGA | TCTTTCAATTTGTCTTTACGCTTTC | Start: 41 |
| Tm | 60.107 | 57.092 | End: 177 |
| Size | 20 | 25 | 137 |
