ssr_primer
Primer Details for NODE_294424_length_531_cov_1.997817 |
|---|
| (GA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGATACGACTGACGAGAGG | GCCAGGACTTTGTCGTCTGA | Start: 301 |
| Tm | 60.042 | 59.966 | End: 522 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GCGATACGACTGACGAGAGG | AGGACTTTGTCGTCTGAGCG | Start: 301 |
| Tm | 60.042 | 60.039 | End: 519 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | CTAGAAGCGGGCTATGGACG | CCTCTCGTCAGTCGTATCGC | Start: 87 |
| Tm | 60.039 | 60.042 | End: 320 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TATACACGAATGCGGTGCGA | GCCAGGACTTTGTCGTCTGA | Start: 389 |
| Tm | 59.899 | 59.966 | End: 522 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | TATACACGAATGCGGTGCGA | AGGACTTTGTCGTCTGAGCG | Start: 389 |
| Tm | 59.899 | 60.039 | End: 519 |
| Size | 20 | 20 | 131 |
(CGA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGATACGACTGACGAGAGG | GCCAGGACTTTGTCGTCTGA | Start: 301 |
| Tm | 60.042 | 59.966 | End: 522 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GCGATACGACTGACGAGAGG | AGGACTTTGTCGTCTGAGCG | Start: 301 |
| Tm | 60.042 | 60.039 | End: 519 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
|---|
| Sequence | CTAGAAGCGGGCTATGGACG | CCTCTCGTCAGTCGTATCGC | Start: 87 |
| Tm | 60.039 | 60.042 | End: 320 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | TATACACGAATGCGGTGCGA | GCCAGGACTTTGTCGTCTGA | Start: 389 |
| Tm | 59.899 | 59.966 | End: 522 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | TATACACGAATGCGGTGCGA | AGGACTTTGTCGTCTGAGCG | Start: 389 |
| Tm | 59.899 | 60.039 | End: 519 |
| Size | 20 | 20 | 131 |
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