Primer Details for NODE_293609_length_249_cov_0.869318 |
|---|
| (T)15c(T)10ctatttctttttcaacttttctttataattttctccctctttttctctcctcg(A)21(T)15ctttttttttc(T)10ctatttttttcta(T)16c(T)10ctaaagccttctttctcatacatttg(T)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCCTCTTTTTCTCTCCTCGA | ACAAATGTATGAGAAAGAAGGCTTT | Start: 58 |
| Tm | 58.907 | 57.47 | End: 202 |
| Size | 22 | 25 | 145 |
| Primer 2 details |
|---|
| Sequence | CTCCCTCTTTTTCTCTCCTCGA | AACAAATGTATGAGAAAGAAGGCTT | Start: 58 |
| Tm | 58.907 | 57.47 | End: 203 |
| Size | 22 | 25 | 146 |
| Primer 3 details |
|---|
| Sequence | CTCCCTCTTTTTCTCTCCTCGA | AAACAAATGTATGAGAAAGAAGGCT | Start: 58 |
| Tm | 58.907 | 57.47 | End: 204 |
| Size | 22 | 25 | 147 |
| Primer 4 details |
|---|
| Sequence | TCTCCCTCTTTTTCTCTCCTCG | ACAAATGTATGAGAAAGAAGGCTTT | Start: 57 |
| Tm | 58.907 | 57.47 | End: 202 |
| Size | 22 | 25 | 146 |
| Primer 5 details |
|---|
| Sequence | TCTCCCTCTTTTTCTCTCCTCG | AACAAATGTATGAGAAAGAAGGCTT | Start: 57 |
| Tm | 58.907 | 57.47 | End: 203 |
| Size | 22 | 25 | 147 |
