Primer Details for NODE_292586_length_274_cov_0.925373 |
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| (A)11ccaac(A)10ttaaataaatgaaaagaaaaaag(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAAGGTCTTTAGTCAGAGTCAACT | ATTTGTGTTTGCTGGTGAGCC | Start: 46 |
| Tm | 58.661 | 59.931 | End: 145 |
| Size | 26 | 21 | 100 |
| Primer 2 details |
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| Sequence | AAAAAGGTCTTTAGTCAGAGTCAAC | ATTTGTGTTTGCTGGTGAGCC | Start: 46 |
| Tm | 57.445 | 59.931 | End: 145 |
| Size | 25 | 21 | 100 |
| Primer 3 details |
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| Sequence | AAAAAGGTCTTTAGTCAGAGTCAACTT | ATTTGTGTTTGCTGGTGAGCC | Start: 46 |
| Tm | 59.17 | 59.931 | End: 145 |
| Size | 27 | 21 | 100 |
| Primer 4 details |
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| Sequence | AAAAGGTCTTTAGTCAGAGTCAACT | TATTTGTGTTTGCTGGTGAGCC | Start: 47 |
| Tm | 58.11 | 59.704 | End: 146 |
| Size | 25 | 22 | 100 |
| Primer 5 details |
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| Sequence | AGGTCTTTAGTCAGAGTCAACTTGG | GTTTATTTGTGTTTGCTGGTGAGC | Start: 50 |
| Tm | 60.222 | 59.791 | End: 149 |
| Size | 25 | 24 | 100 |
