ssr_primer
Primer Details for NODE_292435_length_250_cov_1.293785 |
|---|
| (TGT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGTTGTTGTTGTTGTTGCA | ACAACAAGACAACCAATTAGTGCT | Start: 118 |
| Tm | 58.935 | 59.355 | End: 247 |
| Size | 21 | 24 | 130 |
| Primer 2 details |
|---|
| Sequence | TGCTGTTGTTGTTGTTGTTGC | ACAACAAGACAACCAATTAGTGCT | Start: 117 |
| Tm | 58.935 | 59.355 | End: 247 |
| Size | 21 | 24 | 131 |
| Primer 3 details |
|---|
| Sequence | GCTGTTGTTGTTGTTGTTGCA | ACAACAAGACAACCAATTAGTGCT | Start: 0 |
| Tm | 58.935 | 59.355 | End: 247 |
| Size | 21 | 24 | 248 |
| Primer 4 details |
|---|
| Sequence | GCTGTTGTTGTTGTTGTTGCA | ACACAACAACAATCACAACAACA | Start: 0 |
| Tm | 58.935 | 58.124 | End: 199 |
| Size | 21 | 23 | 200 |
| Primer 5 details |
|---|
| Sequence | TGTTGTAGCTGTAATTGTTGGTGT | GCAACAACAACAACAACAGCA | Start: 38 |
| Tm | 59.115 | 58.935 | End: 137 |
| Size | 24 | 21 | 100 |
(TGT)5tgcaattgtaataattgttgttgttgtagctgtaac(TGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGTTGTTGTTGTTGTTGCA | ACAACAAGACAACCAATTAGTGCT | Start: 118 |
| Tm | 58.935 | 59.355 | End: 247 |
| Size | 21 | 24 | 130 |
| Primer 2 details |
|---|
| Sequence | TGCTGTTGTTGTTGTTGTTGC | ACAACAAGACAACCAATTAGTGCT | Start: 117 |
| Tm | 58.935 | 59.355 | End: 247 |
| Size | 21 | 24 | 131 |
| Primer 3 details |
|---|
| Sequence | GCTGTTGTTGTTGTTGTTGCA | ACAACAAGACAACCAATTAGTGCT | Start: 0 |
| Tm | 58.935 | 59.355 | End: 247 |
| Size | 21 | 24 | 248 |
| Primer 4 details |
|---|
| Sequence | GCTGTTGTTGTTGTTGTTGCA | ACACAACAACAATCACAACAACA | Start: 0 |
| Tm | 58.935 | 58.124 | End: 199 |
| Size | 21 | 23 | 200 |
| Primer 5 details |
|---|
| Sequence | TGTTGTAGCTGTAATTGTTGGTGT | GCAACAACAACAACAACAGCA | Start: 38 |
| Tm | 59.115 | 58.935 | End: 137 |
| Size | 24 | 21 | 100 |
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